Health Diet Plans

Each of us is a miracle of nature, unique and highly complex. Our bodies depend on us to look after them, and keep them running as efficiently and effectively as possible.

The food we eat provides our cells with the nutrients they need to function. Each living cell is a sophisticated unit with a specific role to play in our overall health and vitality. The quality of our diet affects how well our cells function, and has a profound and far reaching effect on our physical and mental well-being.

Good food is one of life’s pleasures, but a poor diet can leave you feeling tired, depressed, bloated, and irritable. Diet is a major contributing factor to many diseases including heart disease and cancer. It can reduce your immunity, and compound existing problems, such as pms, headaches, and hair and skin problems.

The diet plan is mainly based on calorie counting. If you take in fewer calories than you burn fat on a daily basis, then you’ll definitely start losing weight. Although the plans to lose your weight may take in 1200 calories a day. This energy intake is beneficial for almost all types of weight loss due to diet.

Different types of plans are available. But when it comes to weight loss reality very few plans actually deliver. Slim Fast Plan just like Medifast weight loss is such a formula that can actually help you shed those extra pounds. This is one of the most diet programs to effectively promote your to lose fat. It is essentially a balanced plan contains all the essential nutrients to our body. The purpose of this regimen is to focus on a healthy diet and avoid overeating. The plan also provides shakes, juice blends and once a day.

Slim Fast Plan

You must also remember not to push your body too much. Your metabolic system to fail if you want to do that. Note that you need to maintain and take important minerals for your body. Eat moderately and you will be able to keep extra pounds on you. The Diet Solution Program actually suggests you take food very light all day and a normal meal during the night to keep his balance. But if you want to maintain a good shape and weight, then you must continue to keep your balance and physical exercise everyday. Cycling, swimming and hiking are all good health and help shed those extra pounds.

Heart disease for women: The Killer !

Nowadays, heart disease is the number one killer across the globe. It’s no wonder that the disease is now also prevalent in women. Before, heart disease was only associated with men but now it’s also the leading cause of disability and even death in women. Heart disease women should understand that the cardiovascular disease is not only a risk for women but is also oneof the major threats to everyone and your health should be your main priority so it’s a must to not neglect this.

When there’s a narrowing or blockage in your coronary arteries most likely you’ll develop aheart disease. It is where the blood vessels supply blood to the heart. This is also one of themajor reasons why people get heart attacks.

Doctors say that the heart disease of women is different in men. The causes and symptomsof heart disease in both men and women are also different. As a woman gets older, thechance of developing a heart disease also increases. Heart disease women should be takenin a serious manner. It is not a simple disease that can be gone in a minute. In fact, thereare several other factors that can be seen in women and these include: dysautonomias,palpitations, inappropriate sinus tachycardia, and mitral valve prolapse. Yes, the terms are abit vague and hard to comprehend but you can research them on google.
Heart disease women should also be familiar with the causes and the treatments you oughtto expect that are available in the market. For every woman who has a heart disease it isimportant that any changes that may appear to be unfamiliar should be reported to yourdoctor right away. In that way, your doctor can check if the reported changes are linked withyour heart disease. So that it can be treated with the right medicine that you deserve.

In every heart disease women, you should also be vigilant to every symptom that you mayencounter to get rid of increasing heart attacks and early death. The symptoms vary and canbe classified into three: congested, decreased pumping of the heart rate or irregular heartbeat. All of these symptoms are evident when you are suffering a heart disease. We strongly suggest you to read our post with the video for you to recognize those symptoms here: How to recognize women heart attack symptoms from borderline ecg meaning ?

Yes, one who is experiencing heart disease women in life may ruin one’s point of view. Sobefore it can disrupt one’s life it is better if we could prevent the disease than cure it. Asthe old folk say, “prevention is better than cure”. Others suggest that it is through a healthylifestyle habits we could prevent having this heart disease for women.

Reference:

1. About ecg: HTTP://www.healthqas.org

Bringing health and happiness to clients and therapists alike

Bamboo-fusion Massage Therapy: Bringing health and happiness to clients and therapists alike

One of the biggest challenges facing massage therapists is in finding ways to meet clients’ needs, without sustaining the repetitive-use strain so common in the massage therapy profession. Because of the small, repetitive movements used in massage, massage therapists can incur injuries such as carpel tunnel.

Nathalie Cecilia, an experienced massage therapist, is well-versed in these challenges; she has created a wonderful solution with a brand-new spa treatment she calls Bamboo-fusion. The technique was created by Ms. Cecilia In 2004, shortly after she completed her massage therapy training. Cecilia first began using bamboo tools while performing a flowing Swedish massage. The hand-crafted bamboo tools used in her technique are custom-designed, allowing even the smallest therapist to give an effective deep tissue massage while protecting the therapist’s body, hands and wrists.

This trademarked treatment is growing in popularity, and is in high demand in luxury spa settings. The treatment involves the use of bamboo pieces of varying shapes and sizes during a massage treatment. The bamboo pieces function as extensions of the therapist’s fingers, allowing for deeper pressure and greater access, while minimizing strain for the person giving the treatment.

In this method, the bamboo is also brought to a warm temperature, so it can penetrate and release the muscles more quickly. Unlike warm stone massage, the bamboo is easy to heat, without the use of roasters or crock pots. And though the warming method is different, the technique combines very well with warm stone massage. During the Bamboo-fusion massage training classes, students learn to incorporate the use of a few hot stones during a treatment.

The Bamboo-fusion technique uses massage oil or cream in addition to the bamboo tools. Shorter pieces of bamboo work well for smaller areas, such as the areas around the scapula. A 16” bamboo stick is also used in a “criss-cross” fashion that stretches the fascia in multiple directions..

This ecologically sound and sustainable product is made by skilled woodworkers. Bamboo is a highly renewable resource. These tool sets are made with 100% solid bamboo, which is pressed into different shapes and sizes to accommodate the Bamboo-fusion massage routine. The tools, designed for full body massage, hold heat well and are easy and comfortable to use.

In addition to making commercially-available bamboo tool sets, Cecilia also offers massage training to other therapists. Her top-quality professional massage training is valued by high-end spas, which are constantly searching for unique treatments to offer their clients. The training classes are offered in various locations in the United States, and are approved by the state of Florida and the NCBTMB for Continuing Education (CE’s) for license renewal. Classes are offered at numerous training facilities on a regular basis Ms. Cecilia’s home city of Sarasota, Florida. They are also offered in Everett, Durham, and Cincinnati, and at spas and resorts across the country.

To support the educational mission of her work, Cecilia also offers DVD’s and custom bamboo sets for each of her techniques. These tools can be used on the table for full body massage and on the chair while the client is clothed. Cecilia has also created a new French facial massage called Bamboo-Visage which uses a delicate tool set, hand- crafted from bamboo and rattan.

HIV Test Kit

There are many reasons for needing an HIV test kit. You may be a healthcare professional accidentally exposed to someone with HIV. Or, a recent sexual partner may have been diagnosed with HIV, and you want to determine your status with an HIV testing kit. Whatever the cause for your concern, OraWellUSA’s groundbreaking HIV 1 and 2 Antibody Saliva Rapid Screen HIV test kits provide you with a confidential and convenient way to accurately test for HIV in about 10 minutes from the privacy of your home.

Order Our HIV Test Kit Easily and Confidentially

The OraWellUSA HIV 1 and 2 Antibody Saliva Rapid Screen HIV test kit is available for online ordering by clicking the “Buy Now” button located throughout this website. You can use any major credit card for this confidential purchase. We offer a single test kit if you only need one, and those who require more test kits can order in multi-packs of two, three and four. Ordering in multiples reduces the per-kit price. Our HIV test kit, or multiple HIV test kits, will arrive at your home wrapped in discreet packaging.

Take Our HIV Test Kit in About 10 Minutes

Once you receive your OraWellUSA HIV 1 and 2 Antibody Saliva Rapid Screen HIV test kit, simply follow the included step-by-step instructions. From the time you swab your mouth to collect a saliva sample to when the results appear on our test strip, only about 10 minutes will have passed. Our test strip proves easy to read: two pink bands indicate HIV 1 and 2 antibodies in your saliva; one pink band indicates the absence of the antibodies. Compare the 10 minutes or so it takes to use our HIV testing kit with the time involved in testing through your doctor’s office, which often makes you wait days for results, and our HIV test kits are the obvious choice.

Trust in the Accuracy of Our HIV Test Kit

The OraWellUSA HIV 1 and 2 Antibody Saliva Rapid Screen HIV test kit offers 99.98 percent accuracy, according to comparison with external clinical laboratory samples. When our tests returned negative results, they matched the external samples 99.7 percent of the time, while our positive results matched 100 percent of the time. We recommend confirmation with testing provided by a medical professional to anyone who receives a positive result, which also would be the first step toward treatment.

About OraWellUSA and Its Founder

Chief executive officer Dr. Richard M. Blair, M.D., F.A.C.S., established OraWellUSA in 1998 to create a confidential, convenient and simple HIV test kit. His credentials include more than 10 years at the Pasteur Institute in Paris working on the HIV Project. His OraWellUSA HIV 1 and 2 Antibody Saliva Rapid Screen HIV testing kit has received accreditation by the World Health Organization and is recommended by doctors around the world. If you have any reason to be concerned about your HIV status, order one of our test kits today and give yourself peace of mind it provides.

Orawell – Home HIV Test Kits

Possible exposure to HIV is a serious matter, one that you can keep private thanks to OraWellUSA’s groundbreaking HIV 1 and 2 Antibody Saliva Rapid Screen home HIV test kits. Not only do our HIV home test kits come with guaranteed confidentiality, they provide a convenient way to accurately test for HIV in about 10 minutes. You can order a kit online from the privacy of your home using any major credit card, and the kit will arrive in discreet packaging.

Our Home HIV Test Kits Are Easy and Quick to Take

Other HIV home tests kits require you to draw blood. With the OraWellUSA HIV-1 and 2 Antibody Saliva Rapid Screen, you simply collect a saliva sample from your mouth with the included swab, add the sample to a saliva dilution buffer, and apply the diluted saliva sample to our easy-to-read test strip. The results will be ready in just 10 minutes. Two pink-colored bands on the test strip indicate HIV 1 and 2 antibodies in your saliva. One pink-colored band indicates the absence of these antibodies. It’s as easy and quick as that. Were you to take the test through your physician’s office, it would involve providing a blood sample and days, if not weeks, of worry from the time you made your appointment to when you received the results.

Our HIV Home Test Kits Are Accurate

In addition to being confidential, convenient, and quick and easy to take, the OraWellUSA HIV-1 and 2 Antibody Saliva Rapid Screen home HIV test kits deliver 99.98% total accuracy. We know this because we evaluated our samples against external clinical laboratory samples. Negative results were matched 99.7% of the time to external results, with positive results matching 100% of the time. If you do receive a positive result, we recommend confirmation by testing through your physician’s office.

Choose the Home HIV Test Kits Accredited by the WHO

The World Health Organization has accredited the OraWellUSA HIV-1 and 2 Antibody Saliva Rapid Screen HIV home test kits, as has UNICEF. This honor reflects not only the quality and accuracy of the test but the respect given to its inventor. OraWellUSA founder Dr. Richard M. Blair, M.D., F.A.C.S., was a member of the HIV Project team at the Pasteur Institute in Paris from 1982 to 1991. Dr. Blair founded OraWellUSA in 1998 and developed the HIV-1 and 2 Antibody Saliva Rapid Screen home HIV test kits to provide anyone who needs it with access to confidential and accurate HIV testing that is quick and easy to take at home.

Order Your HIV home Test Kits Now

To order one of our OraWellUSA HIV-1 and 2 Antibody Saliva Rapid Screen home HIV test kits, simply click on the “Buy Now” button located throughout this website. We offer our HIV home test kits as a single test and as multi-packs. Packages containing two, three and four test kits reduce the per-kit price, allowing you to test not only yourself but your partner as well.

Hiv Test

New Non-Invasive Home Aids Test

OraWell USA’s ground-breaking home aids test can provide consistent results in as little as 10 minutes. Now, it’s easier than ever to have peace of mind and find out the results of an aids test sooner, without the aggravation or inconvenience of having to visit a doctor’s office or clinic. There is no blood draw required for this aids test, and best of all, the results are completely private.

The OraWell Home Aids Test 1&2 is a completely approved home aids test recommended by doctors and proven to deliver results with more than 99.98% accuracy. The aids test comes with its own supplies so patients have everything they need to collect their own specimen of saliva and a panel to place the specimen onto for analysis. OraWell’s home aids test is easy to use. Just collect a sample of your saliva and place it on the easy-to-use specimen tray.

In about 10 minutes, patients using the home aids test can learn the results, which are produced with a simple positive or negative response. There is no complex scientific analysis of the results or unclear medical terminology to understand, so the OraWell Home Aids Test is appropriate for all users.
Reliable & Confidential Home HIV Test

OraWell USA’s revolutionary, first-of-its kind, home oral HIV test can deliver reliable results in as little as 10 minutes. Now, it’s easier than ever to have peace of mind and find out the results of an HIV test sooner, without the hassle or inconvenience of having to visit a doctor’s office or clinic. There is no blood draw required for the test, and best of all, the results are completely confidential.

The OraWell Oral HIV Rapid Test 1&2 is a fully approved home HIV test recommended by doctors and proven to deliver results with more than 99.98% accuracy. The test comes with supplies so patients can collect their own specimen of saliva as well as a panel to place the specimen onto for analysis. It’s as easy as collecting a sample of saliva and placing it on an easy-to-use specimen tray.

In about 10 minutes, patients using the home HIV test can learn the results, which are produced with a simple positive or negative response. There is no complicated scientific analysis of the results or confusing medical terminology to understand, so the OraWell Oral HIV Rapid Test is suitable for all users.
Scientific Breakthrough: HIV Oral Test

The OraWell HIV-1&2 Antibody Saliva Rapid Screen Test is a chromatographic immunoassay for the detection of antibodies to HIV-1&2 in human saliva. HIV-1&2 antibodies with binding protein are precoated onto membrane as capture reagents. During the testing, a specimen is allowed to react with the colloidal gold reagents which have been labeled with HIV- 1&2 specific antigens. If present in the saliva specimen, the antibodies will cause a pink-colored band to develop on the membrane in proportion to the amount of HIV-1&2 antibodies detected. A negative test result is indicated by an absence of this pink colored band in the test. As a procedural control, a pink-colored band will always appear in the control region of the test, regardless of the presence or absence of the antibodies.

OraWell USA’s revolutionary, HIV saliva test, is very easy to use and does not require the user to understand all the details listed above. The easy to use HIV saliva test can deliver reliable results in as little as 10 minutes. It’s as easy as collecting a sample of saliva and placing it on an easy-to-use specimen tray. HIV testing never got easier!

The OraWell HIV 1&2 Antibody Saliva Rapid Screen Test is a fully approved at-home test recommended by doctors and guaranteed to produce results with more than 99.98% accuracy. The test comes with everything the patient needs to collect their own saliva specimen and analyze it. In about 10 minutes, patients using the test can learn the results, which are produced with a simple positive or negative response. There is no difficult scientific analysis or confusing medical terminology to understand, so the OraWell HIV Saliva Test is suitable for all users.
OraWell HIV Saliva Test: How it Works

The OraWell HIV-1&2 Antibody Saliva Rapid Screen Test is a chromatographic immunoassay for the detection of antibodies to HIV-1&2 in human saliva. HIV-1&2 antibodies with binding protein are precoated onto membrane as capture reagents. During the testing, a specimen is allowed to react with the colloidal gold reagents which have been labeled with HIV- 1&2 specific antigens. If present in the saliva specimen, the antibodies will cause a pink-colored band to develop on the membrane in proportion to the amount of HIV-1&2 antibodies detected. A negative test result is indicated by an absence of this pink colored band in the test. As a procedural control, a pink-colored band will always appear in the control region of the test, regardless of the presence or absence of the antibodies.

OraWell USA’s revolutionary, HIV saliva test, is very easy to use and does not require the user to understand all the details listed above. The easy to use HIV saliva test can deliver reliable results in as little as 10 minutes. It’s as easy as collecting a sample of saliva and placing it on an easy-to-use specimen tray. HIV testing never got easier!

The OraWell HIV 1&2 Antibody Saliva Rapid Screen Test is a fully approved at-home test recommended by doctors and guaranteed to produce results with more than 99.98% accuracy. The test comes with everything the patient needs to collect their own saliva specimen and analyze it. In about 10 minutes, patients using the test can learn the results, which are produced with a simple positive or negative response. There is no difficult scientific analysis or confusing medical terminology to understand, so the OraWell HIV Saliva Test is suitable for all users.

HIV Oral Test Products

We are proud to offer the OraWellUSA “Self-at-Home” HIV oral test to our clients. Here on our website you can easily and securely purchase the self-at-home HIV Antibody test.

This HIV 1&2 test can be used as an at-home, approved, and doctors recommended test that gives you over 99.98% accuracy. They come with collection supplies for collecting your specimen of saliva and a panel which is used for placing the specimen onto for analysis. The HIV test results take only 10 minutes, to be able to read as to whether the subject is Positive or Negative. Of course it is highly recommended to bring or send the specimen to an FDA or EU accredited and certified clinical laboratory for analysis. You should make sure that counseling is available at the laboratory of your choice, should it be necessary to re-confirm the results with the Elisa or Western Blot Test.

OraWellUsa is in the process of gathering all clinical, laboratory results from scores of analyses and experimental, investigative HIV tests which have been conducted in many countries around the world, which show the evidence that our HIV tests are 99.98% accurate, so that we can apply for US approval, as well. This process can take between 9 months to 2 years until the approval is granted.

The FDA and Centers of Disease control recommend that despite the fact that the results of the HIV oral test can be had in 10 minutes, in the house, or else where, they prefer if the individual takes or sends his or her specimen to an FDA acredited laboratory, and let the laboratory determine the HIV test results. It is also very important that should the individual believe that the results could be positive, that he or she should simultaneously consult a competent medical doctor, social worker, or psychologist for the purpose of counseling, at the time of doing the test.

HIV-1/2 Antibody Saliva Rapid Screen Test

Description
The OraWell HIV-1/2 Antibody Saliva Rapid Screen Test is a rapid chromatographic immunoassay for the qualitative detection of antibodies to Human Immunodeficiency Virus type 1 and 2 (HIV-1/2) in human saliva.

How to hiv screening test

How-To – HIV Screening Test

Intended Use

The OraWellUSA HIV-1/2 Antibody Saliva Rapid Screen Test is a rapid chromatographic immunoassay for the qualitative detection of antibodies to Human Immunodeficiency Virus type 1 and 2 (HIV-1/2) in human saliva. All positive specimens must be confirmed with Western Blot or other qualified EIA.

Principle

It has been shown that the acquired immunodeficiency syndrome (AIDS) is caused by viruses which transmitted by sexual contact, transfusion, using contaminated blood products and sharing contaminated needles. HIV-1 and 2 viruses have been isolated from patients with AIDS and AIDS-related complex (ARC), high risk persons for AIDS. HIV-1/2 viruses deplete T helper cells, a subpopulation of T cells for body defense, thus causing AIDS patients susceptible to opportunistic infections and developing malignant tumors. The incidence of specific antibodies to HIV-1/2 is high in AIDS, ARC and persons with high risk for AIDS. The OraWellUSA HIV-1/2 Antibody Saliva Rapid Screen Test was designed to detect antibodies to HIV-1/2 in AIDS patients, ARC or high risk persons and identify any potential donors carrying these antibodies in specimens.

The OraWellUSA HIV-1/2 Antibody Saliva Rapid Screen Test is a chromatographic immunoassay for the detection of antibodies to HIV-1/2 in human saliva. HIV-1/2 antibodies binding protein are precoated onto membrane as capture reagents in the Test region (T). During the testing, specimen is allowed to react with the colloidal gold reagents which have been labeled with HIV-1/2 specific antigens. Antibodies to HIV-1/2, if present, a pink colored band will develop on the membrane in proportion to the amount of HIV-1/2 antibodies present in the saliva specimen. Absence of this pink colored band in the test region (T) suggests a negative result. to serve as a procedural control, a pink colored band in the control region (C) will always appear regardless of the presence or absence of antibodies to HIV-1/2 in human saliva.

Materials Provided

Each OraWellUSA HIV-1/2 Antibody Saliva Rapid Screen Test contains:

  1. 1 Foil Pouche. Each pouch contains one device (assay test strip) and one desiccant.
  2. 1 Dropper bottle of Saliva Dilution Buffer (1ml per bottle).
  3. 1 Saliva Collector.
  4. 1 Package Insert.

Materials Required but Not Provided

  1. Timer or clock
  2. Disposable gloves

Storage and Stability

The test device is to be stored refrigerated or at room temperature (2-30°C) under dry conditions for the duration of its shelf life. The test device is stable through the expiration date printed on the sealed pouch. The test device must be kept sealed in the pouch until use. DO NOT FREEZE!

Precaution

  1. For in vitro diagnostic use only.
  2. Do not use a test device beyond the expiration date imprinted on the back of the foil pouch.
  3. Nothing should be placed into the mouth of the subject for at least 10 minutes prior to saliva collection. Those include food, drink, tobacco products or any other materials.
  4. Avoid cross contamination of saliva samples by using a new saliva Collector and Dropper bottle for each sample.
  5. Saliva specimens may be potentially infectious. Avoid contacting with skin by wearing gloves and proper laboratory attire. Properly handle and dispose of all used test devices in an approved biohazard container.
  6. All specimens should be treated as if capable of transmitting diseases. Observe established precautions against microbiological hazards throughout the procedure and follow the standard procedures for proper disposal of specimens.
  7. Wear protective clothing such as laboratory coats, disposable gloves or eye protector when specimens are being tested.
  8. Immediately clean up any spillage of materials with a 1:10 dilution of 5% sodium hypochlorite. Autoclave any contaminated materials for 60 minutes at 121°C before disposal. All liquid waste should be treated with 5% sodium hypochlorite solution (the final concentration should not be lower than 0.5% sodium hypochlorite) for 30 minutes before disposal.
  9. High humidity and temperature can adversely affect results.

Specimen Collection and Storage

The OraWellUSA HIV-1/2 Antibody Saliva Rapid Screen Test is designed for the use of buffered human fresh saliva as the specimen. Collecting specimen must follow standard clinical procedure. If specimens are to be shipped, they should be packed in compliance with federal regulations covering the transportation of etiologic agents. Do not place anything into the mouth including food, drink, gum, or tobacco products for at least 10 minutes prior to collection of oral fluid specimen.

  1. Remove the saliva collector out of the pouch.
  2. Pull out the collection swab from the collection chamber.
  3. Place the collection swab into mouth and gently move around root of the teeth for about 5 minutes to let sufficient saliva be collected in the swab.
  4. Remove the swab out of the mouth and place it inside the chamber.
  5. Push the swab to squeeze out 1ml saliva to a dropper bottle which contains 1ml saliva dilution buffer. Make sure the total volume is around 2ml. Mixing well before using.

*Saliva specimens can be kept at room temperature if a test is performed within 24 hours after collection. The specimens must be stored at 2-8°C for two weeks before using. If the test is not performed within 2 weeks, saliva specimens should be frozen for prolonged storage. Frozen specimens must be completely thawed and mixed well prior to testing. Avoid repeatedly freezing and thawing of specimens.

Assay Procedure

  1. Do not open foil pouch until ready to begin testing. Refrigerated test devices should be allowed to come to room temperature (15- 28°C) before opening the pouch.
  2. Remove the device from the protective pouch and label the device with specimen identification. The product is sensitive to the presence of moisture. After opening the package, the test device should be used immediately.
  3. Add 6 drops (250μl) of fresh saliva specimen to the sample well.
  4. Read the result within 10 minutes. Observe the colored band developed over the control region indicating the assay is complete.

IMPORTANT: In order to prevent an incorrect reading, do not read the test results after 15 minutes. If the test is read after 15 minutes, the intensity of the colored bands may change or a new band may appear in test region (T). to avoid confusion, discard all test devices after interpreting results.

Interpretation of Result

POSITIVE: Presence of two visible, pink-colored bands, one in the control region (C) and the other in test region (T), indicates presence of HIV-1/2 antibodies in sample.

NEGATIVE: Presence of a single colored band in the control region (C) indicates the absence of HIV-1/2 antibodies or that the concentration of antibodies in sample is below the detection cut-off level.

INVALID: Results are invalid if no band appears in the control region, or a band appears only in the test region after 10 minutes. An invalid result may be due to improper assay procedures or damage to the device. The assay is inconclusive and the specimen should be re-tested using a new test device.

NOTE: Do not interpret results after 15 minutes. The test band intensity may be weaker or stronger than that of the control band, but a very faint band in the test region indicates that the concentration of HIV-1/2 antibodies in the sample is lower than the detection cut-off level. The sample should be re-tested or confirmed with a more specific method before a positive determination is made.

After Testing

The saliva specimens may be potentially infectious. Avoid contacting with skin by wearing gloves and proper laboratory attire. Properly handle and dispose of all test devices in an approved biohazard container. Residual sample should be disposed of in a medically approved manner after completion of all testing.

Quality Control

The OraWellUSA HIV-1/2 Antibody Saliva Rapid Screen Test has a built-in process Control Region. A pink band should always appear in this region regardless of the presence or absence of antibodies to HIV-1/2 in human saliva.. This pink process Control band verifies: 1) that sufficient sample volume was added, 2) that proper flow was obtained, and 3) that the reagents worked properly. If this band is missing, the test was not performed correctly or failed to function correctly. Such a test is inconclusive and the specimen should be re-tested using a new test device. Good laboratory practice requires use of control materials to ensure proper test device performance and reliability. Quality control standards are available for the validation of device functionality from certified labs. When testing the quality control standards, use the same assay procedure as with a oral fluid sample. It is recommended that control specimens be used for each new lot of kits.

Assay Comparisons & Equivalency

Accuracy and equivalency comparisons of OraWellUSA HIV-1/2 Antibody Saliva Rapid Screen Test were evaluated against 600 individual external WB-certified clinical laboratory samples. The results have been tabulated below.

OraWellUSA Rapid Screen Test vs. WB

WB HIV 1/2 Test(+) HIV 1/2 Test(-) Rowtotals
(+) 300 0 300
(-) 1 299 300
Col.totals 301 299 600

When compared to WB, the percent agreement with OraWellUSA HIV-1/2 Antibody Saliva Rapid Screen Test, positive samples were 300/300 or 100%. Negative samples recovered at 299/300 or 99.7%, while the overall relative accuracy obtained was 599/600 or 99.8%.

Limitations

The OraWellUSA HIV-1/2 Antibody Saliva Rapid Screen Test procedure and the interpretation of results must be followed closely. A test result read after 15 minutes may not be consistent with the original reading obtained within the 15 minutes test period. This assay is designed for testing antibodies against HIV-1/2 in human saliva. Any results derived from the test of other body fluids may not be interpreted correctly based on the current criteria. Other body fluids and pooled samples are not recommended in this assay.

The OraWellUSA HIV-1/2 Antibody Saliva Rapid Screen Test is designed to detect antibodies to HIV-1/2 in saliva and thus is useful in screening blood donated for transfusion and further manufacture, in establishing prior infection of HIV or in evaluating patients with AIDS symptoms and signs. For positive specimens, it is recommended that more specific supplemental test should be conducted and clinical evaluation on patient’s situation should be performed before final conclusion is made. HIV-1/2 rapid testing alone cannot be used to diagnose AIDS even if the antibodies against HIV-1/2 are present in patient’s saliva. The test result will only indicate the qualitative level of antibodies of HIV 1/2 in the specimen and should not be used as the sole criteria for the diagnosis of HIV. Even if the test results are positive, further clinical evaluation should be considered with other clinical information available to the physician. A negative result does not rule out infection by HIV because the antibodies to HIV may be absent or may not be present in sufficient quantities to be detected at a very early stage of infection or could result from improper sample collection.

References

  1. M Popovic, MG Sarngadharan, E Read, and RC Gallo. Detection, isolation and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-Aids. Science 1984, 497-500.
  2. RC Gallo, SZ Salahuddin, M Popovic, et al. Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS . Science 1984, 224:500-503.
  3. JW Curran, WM Morgan, AM Hardy, et al., The epidemiology of AIDS: current status and future prospects. Science 1985, 229:1352-1357.
  4. Clavel, F. HIV-2, The West African AIDS virus. AIDS 1987, 1:135-140.
  5. P Piot, FA Plummer, FS Mhalu, et al. AIDS: an international perspective, Science 1988, 239:573-579.
  6. Provisional Public Health Service Interagency recommendations for screening donated blood and plasma for antibody to the virus causing Acquired Immunodeficiency Syndrome. MNWR. 1985,34: 1-5.